Cell free supernatant, Drug resistance, Lactobacillus plantarum, S. flexneri
Citation Information :
Garg N, Rishi P, Goad J, Bharrhan S. Antibacterial and immunomodulatory effect of cell free supernatant of Lactobacillus plantarum against Shigella flexneri. J Gastrointest Infect 2013; 3 (1):33-40.
Background & Objectives: Shigellosis is endemic throughout the world causing great deal ofmorbidity andmortality. Emergence of antibiotic resistance and lack of vaccine against Shigella, necessitates exploitation of alternative strategies to combat Shigella infection. The present studywas undertaken to evaluate the effect of cell free supernatant (CFS) from Lactobacillus plantarum (L. plantarum) against Shigella flexneri (S. flexneri).
Methods: The effect of CFS from Lactobacillus plantarum against S. flexneri was studied in terms of agar well diffusion assay, time kill assay, adherence inhibition andmacrophage functions.
Results: It was found that CFS inhibits S. flexneri as indicated by zone of inhibition and continuous decrease in the CFU/ml for 16 hours of incubation period. A decrease in the number of Shigella cells adhering to mouse intestinal cells was also observed in the presence of CFS. Cell free supernatant could significantly decrease the levels of malondialdehyde (MDA) and nitrite. Levels of lactate dehydrogenase (LDH) and Tumor necrosis factor-α (TNF-α) were almost restored in the presence of CFS. The study revealed correlation between LDH and MDA levels, as well as between nitrite and TNF-α levels suggesting the immunomodulatory effects of CFS in addition to its antibacterial property against Shigella.
Interpretation and Conclusions: Regular intake of probiotic food supplements may prove to be beneficial against enteric infections due to sustained release of antimicrobials.
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