Journal of Gastrointestinal Infections

Register      Login

VOLUME 6 , ISSUE 1 ( 2016 ) > List of Articles

BRIEF COMMUNICATION

SSU rRNA target amplification of intestinal microsporidia: Asensitive diagnostic tool for accurate estimate of its prevalence

Ashish Goel, Rumpa Saha, Stuti Kaushik, V G Ramachandran

Keywords : Diarrhea, Encephalitozoon, Enterocytozoon bieneusi, HIV, microsporidia

Citation Information : Goel A, Saha R, Kaushik S, Ramachandran VG. SSU rRNA target amplification of intestinal microsporidia: Asensitive diagnostic tool for accurate estimate of its prevalence. J Gastrointest Infect 2016; 6 (1):50-53.

DOI: 10.5005/jp-jogi-6-1-50

License: CC BY-SA 4.0

Published Online: 01-12-2016

Copyright Statement:  Copyright © 2016; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

The diagnosis of intestinal microsporidiosis has traditionally relied on light microscopy. Western literature shows PCR to be more sensitive and specific. The present study was conducted to assess the prevalence of enteric microsporidiosis in HIV seropositive patients using PCR. Five percent stool samples were found to be positive for microsporidia by pan microsporidia primers and found to be Enterocytozoon bieneusi on amplification using species specific primers. Microsporidia is grossly under-reported in our country and there is a dire need to institute measures to detect this organism particularly in HIV infected individuals to abate morbidity and mortality due to this organism.


PDF Share
  1. Matsubayashi H, Koike T, Mikata T, Hagiwara S. A case of Encephalitozoon like body infection in man. Arch Pathol. 1959;67:181-7.
  2. Orenstein JM, Dieterich DT, Kotler DP. Systemic dissemination by a newly recognized intestinal microsporidia species in AIDS. AIDS. 1992;6:1143-50.
  3. Desportes I, Le Charpentier Y, Galian A, Bernard F, Cochand- Priollet B, Lavergne A et al. Occurrence of a new microsporidian: Enterocytozoon bieneusi n.g., n.sp., in the enterocytes of a human patient with AIDS. J Protozool. 1985;32:250-4.
  4. Zhu X, Wittner M, Tanowitz HB, Kotler D, Cali A, Weiss LM. Small subunit rRNA sequence of Enterocytozoon bieneusi and its potential diagnostic role with use of the polymerase chain reaction. J Infect Dis. 1993;168:1570-5.
  5. Satheesh KS, Ananthan S, Lakshmi P. Intestinal parasitic infections in HIV-infected patients with diarrhoea in Chennai. Indian J Med Microbiol. 2002;20:88-91.
  6. Saigal K, Sharma A, Sehgal R, Sharma P, Malla N, Khurana S. Intestinal microsporidiosis in India: a two year study. Parasitol Int. 2013;62:53-6.
  7. Gupta M, Sinha M, Raizada N. Opportunistic intestinal protozoan parasitic infection in HIV positive patients in Jamnagar, Gujrat. SAARC J Tuber Lung Dis HIV/AIDS. 2008;5:21-4.
  8. Dalvi S, Mehta P, Koticha A, Gita N. Microsporidia as an emerging cause of parasitic diarrhoea in HIV seropositive individuals in Mumbai. Bombay Hosp J. 2006;48:592-7.
  9. Kairon R. Study on opportunistic enteric parasites in HIVseropositive adult patients hospitalized for diarrhoea. National AIDS Research Institute. Annual Report 2003. 2003-2004.
  10. Ghoshal U, Khanduja S, Pant P, Prasad KN, Dhole TN, Sharma RK et al. Intestinal microsporidiosis in renal transplant recipients: Prevalence, predictors of occurrence and genetic characterization. Indian J Med Microbiol. 2015;33:357-63.
  11. Espern A, Morio F, Miegeville M, Illa H, Abdoulaye M, Meyssonnier V et al. Molecular study of microsporidiosis due to Enterocytozoon bieneusi and Encephalitozoon intestinalis among human immunodeficiency virus-infected patients from two geographical areas: Niamey, Niger, and Hanoi, Vietnam. J Clin Microbiol. 2007;45:2999-3002.
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.