Probiotic Lactobacillus rhamnosus GG inhibits the adhesion of Giardia intestinalis to murine enterocytes: An in vitro study
Nisha Goyal, Vivek Sharma, Geeta Shukla
Giardiasis, mouse enterocytes, probiotic, scanning electron microscopy
Citation Information :
Goyal N, Sharma V, Shukla G. Probiotic Lactobacillus rhamnosus GG inhibits the adhesion of Giardia intestinalis to murine enterocytes: An in vitro study. J Gastrointest Infect 2016; 6 (1):45-49.
Background and Objectives: Giardiasis mainly affects young children causing diarrhea, malnutrition and growth retardation. Due to adverse effects of antiprotozoal treatment such as low compliance with drug therapy, reinfestation, occurrence of resistant strains, headache and metallic taste, the use of natural live bacteriotherapy has been studied. The study was designed to investigate in vitro the colonizing ability of probiotic Lactobacillus rhamnosus GG (LGG) viz-a-viz its ability to inhibit the adherence of Giardia trophozoites to murine enterocytes under conditions simulating the intestinal environment.
Materials and methods: Murine enterocytes were harvested and incubated with Giardia trophozoites either prior or simultaneously with probiotic LGG to assess the adhesion using scanning electron microscopy.
Results: It was observed that 15% of Giardia trophozoites adhered to enterocytes at 37°C in Hank\'s Balanced Salt Solution, after 1h of incubation. However, coincubation of murine enterocytes with probiotic LGG either 30 min prior or simultaneously with Giardia trophozoites led to 23-27% reduction in the adherence of Giardia trophozoites compared with 46% adherence in the absence of LGG. Further, scanning electron microscopy also showed in vitro inhibition of Giardia trophozoites to murine enterocytes due to probiotic supplementation.
Iterpretations and conclusion: The data suggest the colonizing ability of probiotic LGG to murine enterocytes that modulates murine giardiasis mainly by displacing the Giardia trophozoites.
Savioli L, Smith H, Thompson A. Giardia and Cryptosporidium join the 'Neglected Diseases Initiative'. Trends in Parasitol. 2006;22:203-8.
Geurden T, Vercruysse J, Claerebout E. Is Giardia a significant pathogen in production animals? Exp Parasitol. 2010;124:98- 106.
Pérez PF, Minnaard J, Rouvet R, Knabenhans C, Brassart D, Antoni GL et al. Inhibition of Giardia intestinalis by extracellular factors from lactobacilli: An In vitro study. App Envir Microbiol. 2001;67:5037-42.
Cotton JA, Beatty JK, Buret AG. Host parasite interactions and pathophysiology in Giardia infections. Int J of Parasitol. 2011;41:925-33.
Shukla G, Devi P, Sehgal R. Effect of Lactobacillus casei as a probiotic on modulation of giardiasis. Dig Dis and Sci. 2008;53:2671-9.
Shukla G, Kaur T, Sehgal R, Rishi P, Prabha V. Protective potential of Lactobacillus acidophilus, a biotherapeutic agent in murine giardiasis. Cent Euro J Med. 2010;5:456-63.
Saarela M, Mogensen G, Fonden, Matto RJ, Mattila-Sandholm T. Probiotic bacteria: safety, functional and technological properties. J Biotechnol. 2000;84:197-215.
Collado MC. Probiotic strains and their combination inhibit in vitro adhesion of pathogens to pig intestinal mucosa. Curr Microbiol. 2007;55:260-5.
Goyal N, Tiwari RP, Shukla. G. Lactobacillus rhamnosus GG as an effective probiotic for murine giardiasis. Interdiscipl Perspect on Infect Dis. 2011;doi:10.1155/2011/795219.
Goyal N, Shukla G. Probiotic Lactobacillus rhamnosus GG modulates the mucosal immune response in Giardia intestinalis infected BALB/c mice. Digest Dis and Sci. 2012;doi: 10.1007/S10620-012-2503.
Bergmeyer HU. Methods of enzymatic analysis. Acad Press, Inc, New York 1963.
Céu Sousa M, Gonçalves CA, Bairos VA, Poiares-da-Silva J. Adherence of Giardia lamblia trophozoites to Int-407 Human intestinal cells. Clin Vacci Immunol. 2001;8:258-65.
Alander M, Satokari R, Korpela R, Saxelin M, Vilpponen-Salmela T, Mattila- Sandholm T et al. Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG after oral consumption. App Envir Microbiol. 1999;65:351-4.
Inge PM, Edson CM, Farthing MJ. Attachment of Giardia lamblia to rat intestinal epithelial cells. Gut. 1988;29:795-801.
Katelaris PH, Naeem A, Farthing MJ. Attachment of Giardia lamblia trophozoites to a cultured human intestinal cell line. Gut. 1995;37:512-28.
Bernet MF, Brassart D, Neeser JR Servin AL. Lactobacillus acidophilus La1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria. Gut. 1994;35:483-9.
Reid G, Howard J, Gan BS. Can bacterial interference prevent infection? Trends in Microbiol. 2001;9:424-8.
Servin AL, Coconnier MH. Adhesion of probiotic strains to the intestinal mucosa and interaction with pathogens. Best Pract Res Clin Gastroenterol. 2003;17:741-54.