Citation Information :
Lahori M, Rishi P, Tiwari RP. Stability of antimicrobial activity of cryptdin-2 against selected pathogens under physiological conditions. J Gastrointest Infect 2012; 2 (1):30-37.
Background and Objective: An initial step prior to clinical development of any therapeutically active peptide is to evaluate its stability under physiological conditions. As cationic antimicrobial peptides have been reported to lose their activity under physiological conditions, present study was done to evaluate the stability of antimicrobial activity of cryptdin-2 (a Paneth cell antimicrobial peptide) against Salmonella Typhimurium, Yersinia enterocolitica and Staphylococcus aureus in the presence and absence of physiological concentrations of bile salts, monovalent and divalent cations, trypsin as well as at various p H values.
Methods: The antimicrobial activity of cryptdin-2 under various physiological conditions against Salmonella Typhimurium NCTC 74, Yersinia enterocolitica and Staphylococcus aureus was evaluated by use of a modified broth dilution technique
Results: Interestingly, the activity of the peptide against the Gramnegative strainswas augmented by bile salts while no change in the activity against S. aureus was observed. Though there was a decrease in activity with increasing concentrations of metal ions, the activity was not completely lost. The peptide was able to sustain its activity against all the three test strains at physiological concentrations of trypsin. At p H8, no change in activitywas observed against Y. enterocolitica and S. Typhimurium while it was found to be reduced against S. aureus.
Interpretation and Conclusion: The study provides data showing the stability of the peptide under the physiological conditions and indicates towards the possibility of developing it as an alternate strategy to combat bacterial pathogens.
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